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The study was conducted on invertebrate population assemblage on three locally available substrates (Bamboo: Bambusa vulgaris; Mehogini: Swietenia macrophylla and Rain tree: Samanea saman) as well as their diversity in a culture pond at Dhaka, Bangladesh. It was observed that a total of 45 taxa of zooperiphyton were recorded, of which, 10 taxa belonged to protozoans, 19 rotifers, two copepods, eight cladocerans, three insects and one taxon from ostracods, oligochaetes and nematodes. Among all three types of substrates, the mean value of zooperiphyton abundance was 28789.07±5029.90 individual m-2. Protozoa (41%) and Rotifera (41%) were the most dominant groups of total zooperiphyton abundance. Among protozoans, Epistylis sp. (78.60%) and Arcella sp. (8.69%); among rotifera, Rotaria neptunia (56.31%) and Brachionus angularis (9.09%); among copepoda, naupleus (59.46%) and Cyclops sp. (31.66%); among cladoceran, Alonella sp. (36.91%) and Alona sp. (18.10%),; among ostracods, Cypris sp. (100%); Among insects, Carpophaga sp. (55.88%) and Chironomus larva (37%); among oligocheates, Nais sp. (100%) were found to be the most dominant species. The abundance and zooperiphyton community were well diversified and assemblage in Bamboo, Mehogoni and Rain tree substrate in National museum pond. The research also indicate that need attention followings: Epistylis sp. for casual agents of fish diseases; Nais sp. as pollution signal producing periphytic oligocheats and nematodes zooperiphyton have significant research arena of freshwater nematodes community. Therefore, further extensive study is needed to explore the knowledge of zooperiphyton in relation to limnological, human health, fish diseases and to determine of urban water status.